Cytotoxicity of new psychoactive substances and other drugs of abuse studied in human HepG2 cells using an adopted high content screening assay. New psychoactive substances (NPS) are still an emerging issue in clinical and forensic toxicology. Information about their cytotoxic potential is limited or even unavailable before distribution and thus their intake can be of high risk for consumers. The present study showed strong cytotoxic potential for the NPS 5F-PB-22 and MDAI, moderate effects for MDMA, MDPV, methylone, cathinone, 4-MEC, and mephedrone, and no toxic effects for methamphetamine. To assess the metabolic suitability of HepG2 cells under the chosen conditions, cell culture supernatants were analyzed by liquid chromatography-high resolution-tandem mass spectrometry. Metabolites were merely detected for lipophilic drugs such as 5F-PB-22 and MDPV and in addition with a much lower abundance in comparison to the parent compound but the study only allowed a qualitative look for metabolites and the used liver cell line might not ideal when considering metabolism.
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Tag
abuso
addiction
alcohol
alcol
analysis
arterie
brain
buprenorphine
cannabis
cell
coca
Cocaine
depression
Disease
drugs
EMCDDA
eroin
espad
EU
Fentanyl
hepatitis
hiv
Inhalants
Methylphenidate
naltrexone
Neurology
nida
opioid
opioids
Prevention
psychoactive
psychoactive substances
Research
schizophrenia
study
THC
tobacco
UNODC
virus